Rutgers / Environmental & Occupational Health Sciences Institute (EOHSI)



Debra Laskin, Ph.D.

Associate Director:

Carol Gardner, Ph.D.

Associate Director:

Vasanthi Sunil, Ph.D.

Lab Manager:

Theresa Hyejeong Choi, M.S.

Technical Support:

Jessica Cervelli


August 2015:

The new CytoFLEX Cytometer with 2 lasers and up to 6 fluorescence colors is available in the facility. Please click here for the information of the CytoFLEX.


April 2015:

We are launched FluoroFinder program for multicolor immunofluorescence to find fluorochromes and antibodies for your panel design.

Please click here to go to FluoroFinder.


Oct. 2012:

The new Gallios Flow Cytometer with 10 colors, 3 lasers is available.


Jan. 2010:

Core Facility is please to introduce the instrument : BD FACSArray Bioanalyzer.


Jan. 2009:

The new state-of-the-art instrument, Beckman Coulter MoFlo XDP Cell Sorter, is avaliable for BL2 cell sorting.














Flow cytometry is a laser-based technology that permits rapid and precise multiparameter analysis of individual cells and particles from within a heterogeneous population or tissue section. Using the instruments in this facility, information on the physical and fluorescence properties of individual cells or organelles can be obtained, as well as their tissue and subcellular localization.

The Flow Cytometry/Cell Sorting & Confocal Microscopy Core Faciltiy provides investigators with rapid and precise multiparameter analysis of cells, particles, and tissue samples. Laser based cytometry provides a high intensity light source for efficient and broad range illumination of fluorescent probes.

The instruments are linked to computer work stations for rapid data analysis. Typically, investigators, their students, and/or technicians work with technical experts in the core facility to analyze samples and the resultant data, thereby gaining first-hand experience in data acquisition and analysis. The staff also works closely with investigators to design experiments and protocols for optimal utilization of the facility. A variety of different services currently available are listed below. Experimental protocols are available for each of these techniques to guide investigators. New methods are routinely being established and tested.

services include

  • ImmunophenotypingSP
  • DNA and RNA content
  • Cell-cycle distribution and Tumor-ploidy determination
  • Apoptosis assays (Annexin V, TUNEL assay, and Caspase activity, etc.)
  • Cell proliferation assay
  • Cell function measurements (i.e., viability, oxidative metabolism, and phagocytosis)
  • Cell biochemistry (i.e., intracellular pH, calcium mobilization, mitochondria membrane potential, microviscosity, and glutathione content)
  • Analysis of fluorescent protein (GFP, YFP, and RFP) expression
  • Side population analysis for stem cell research
  • Fluorescence imaging of individual cells and tissue samples
  • Cell sorting and cloning
  • IVIS Small Animal Imaging

The Facility is also equipped with a laser-based scanning confocal microscope (Leica TCS SP5). This instrument can be used to:

  • Conduct advanced imaging of tissue sections, cell monolayers and single cells.
  • Quantitate Ca++, pH, and membrane potential transients of viable cells.
  • Conduct fluorescent in situ hybridization (FISH) analysis in tissue sections or single cells.
  • Collect 3-D information from complex samples/reconstruct 3-D images for extended analysis.
  • Image subcellular processes as they occur.