Rutgers / Environmental & Occupational Health Sciences Institute (EOHSI)

News

April 2015:

We are launched FluoroFinder program for multicolor immunofluorescence to find fluorochromes and antibodies for your panel design.

Please click here to go to FluoroFinder.

 

Oct. 2012

The new Gallios Flow Cytometer with 10 colors, 3 lasers is available.

 

Jan. 2010:

Core Facility is please to introduce the instrument : BD FACSArray Bioanalyzer.

 

Jan. 2009:

The new state-of-the-art instrument, Beckman Coulter MoFlo XDP Cell Sorter, is avaliable for BL2 cell sorting.

 

 

 

 

 

 

 

 

Rutgers

EOHSI

CEED_NIEHS

Leica Tcs SP5 Confocal Microscope

DAPI+AF488+AF568

This is a laser based confocal system that permits measurements of fluorescently labeled tissue sections, individual cells, or particles adhered to matrices over a wide selection of visible wavelengths.

The instrument is equipped with 5 air cooled lasers allowing for excitation wavelengths of 405, 458, 476, 488, 496, 514, 561, 594 and 633 nm.

Analysis of immunofluorescence as well as functional and biochemical kinetic studies can be performed on viable cell samples. The instrument is equipped with a Ludin cube live cell chamber allowing for temperature control allowing for long term observation of viable cell cultures. Intracellular distribution of fluorescence can also be quantified. The instrument has extremely high sensitivity and can differentiate between closely emitting chromophores.

In addition, the Leica TCS SP5 features digital resolution of 64 megapixels X12 bits per channel with 4 detector channels and can measure real time response of time-critical sequences due to its fast scan speed.

Leica TCS SP5 Specifications:MP_DAPI+RhoPhalloidin

    • Microscope:
        • Leica DMI6000CS (inverted)
        • Live cell chamber (Ludin cube)
        • Motorized scanning stage
    • 5 air cooled lasers:
        • 405nm Diode laser
        • Argon laser(458, 477, 488, 496, 514 nm)
        • 561nm Diode laser
        • HeNe 594 nm
        • HeNe 633 nm
    • Objectives:
        • 10x/0.40
        • 20x/0.70
        • 40x/1.25-0.75 oil
        • 63x/1.20 water
        • 63x/1.40 oil
        • 63x/1.30 glycerin

For the information of the cell culture dishes/or chambers for Leica SP5 confocal microscopy: click here

To download the Leica software (LAS AF Lite-version 2.3.0): Click here

TRITC

 

 

Location: Pharmacy Building, Room 003

 

 

mouse ear