Rutgers / Environmental & Occupational Health Sciences Institute (EOHSI)

News

April 2015:

We are launched FluoroFinder program for multicolor immunofluorescence to find fluorochromes and antibodies for your panel design.

Please click here to go to FluoroFinder.

 

Oct. 2012

The new Gallios Flow Cytometer with 10 colors, 3 lasers is available.

 

Jan. 2010:

Core Facility is please to introduce the instrument : BD FACSArray Bioanalyzer.

 

Jan. 2009:

The new state-of-the-art instrument, Beckman Coulter MoFlo XDP Cell Sorter, is avaliable for BL2 cell sorting.

 

 

 

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Rutgers

EOHSI

CEED_NIEHS

Beckman Coulter Cytoflex Cytometer

 

 

 

 

 

 

Beckman Coulter CytoFLEX Cytometer is a compact benchtop flow cytometer for research use. CytoFLEX flow cytometer can acquire up to 8 parameters on a single sample excited by the 488nm and 638nm lasers, up to 6 fluorescence colors, plus forward and side scatter data channels. It can be upgradable adding a 405nm laser and up to 13 fluorescence colors in the future.

CytoFLEX Cytometer uses the sample peristaltic pump to take sample and calibrates sample volume delivery for absolute cell counts without using beads. It can process samples at flow rate between 10 uL and 240 uL per minute providing high sensitivity and adjustable flow rates.

The CytExpert software provides 7 decade of data display and the event collection rate can be up to 30,000 events/second.

Sample Input Format: Single tube loader format - 5mL (12x75mL) polypropylene tubes, Microcentrifuge 1.5mL and 2mL tubes

CytoFLEX Laser Specification

  Lasers Power output
  Blue laser, 488nm 50 mW
  Red laser, 638nm 50 mW

 

CytoFLEX Configuration

CytoFLEX_Configuration

 

Location:  EOHSI Building, Room 347

Available software for analyzing data:

CytExpert software allows visualization of data acquisition and data analysis of the results. CytExpert software can be installed on your desktop computer and analyze your data on your own. The data is created as FCS 3.0 format and can be analyzed by Kaluza Analysis Software or other third party software.