Rutgers / Environmental & Occupational Health Sciences Institute (EOHSI)

News

April 2015:

We are launched FluoroFinder program for multicolor immunofluorescence to find fluorochromes and antibodies for your panel design.

Please click here to go to FluoroFinder.

 

Oct. 2012

The new Gallios Flow Cytometer with 10 colors, 3 lasers is available.

 

Jan. 2010:

Core Facility is please to introduce the instrument : BD FACSArray Bioanalyzer.

 

Jan. 2009:

The new state-of-the-art instrument, Beckman Coulter MoFlo XDP Cell Sorter, is avaliable for BL2 cell sorting.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Rutgers

EOHSI

CEED_NIEHS

beckman Coulter Gallios Flow cytometer

Gallios transparent (photo from Beckman Coulter)

The Gallios flow cytometer is an ultra sensitive high speed flow cytometer with 3 solid state lasers and 10 fluorescence colors. Its high stability and sensitivity allow very dim and rare populations to be accurately analyzed. Gallios has a customized forward scatter to achieve the most accurate size measurement and optimized electronics for high resolution and fast, accurate, and efficient processing.

The Gallios is the next generation flow cytometer from the existing Beckman Coulter FC500 flow cytometer in the Core facility. Data acquired by Gallios can be analyzed with Kaluza analysis software, which is available for users to analyze data on their own computers. In addition, data collected on the FC500 instruments can also be analyzed on the new Kaluza software.

The instrument can analyze particle size ranges from 0.4 µm to 40 µm diameter.

Maximum Acqusition Rate: 25,000 events per second

Gallios Flow Cytometer Specifications:

  Lasers Power output
  Blue Solid State Diode, 488nm 22 mW
  Red Solid State Diode, 638nm 25 mW
  Violet Solid State Diode, 405nm 40 mW

 

Excitation
Detectors
Emission
Fluorochromes
488nm
FL1
525/20nm
FITC, Alexa Fluor 488, GFP, YFP, CFSE, Rhodamine123, Vybrant Green
488nm
FL2
575/20nm
PE, DsRed, Vybrant orange
488nm
FL3
620/30nm
PE-Texas Red(ECD), PI, PE-CF 594
488nm
FL4
675/20nm

or

695/30nm

PE-Cy5, PerCP, 7AAD

 

PE-Cy5.5, PerCP-Cy5.5, PerCP-eFluor 710
488nm
FL5
755LP nm
PE-Cy7
633nm
FL6
660/20nm
APC, Alexa Fluor 647
633nm
FL7
725/20nm
Alexa Fluor 700
633nm
FL8
755LP nm
APC-Cy7, APC-Alexa750, APC-Alexa 770
405nm
FL9
450/50nm
Pacific Blue, Cascade Blue, Brillant Violet 421, eFluor 450, Alexa Fluor 405
405nm
FL10
550/40nm
Cascade Yellow, Krome Orange, Pacific Orange, Alexa Fluor 430

 

Location:  EOHSI Building, Room 347

Available software for analyzing data:

  • Kaluza Analysis Software is available.
  • You can analyze your data on your computer using Kaluza analysis software. First, download the one-month free demo Kaluza software on your computer by clicking here. Second, ask us to borrow a dongle for your computer to use the Kaluza analysis software.

    To download of Kaluza Instruction : click here

    ModFit
  • ModFit LT, Verity Software House, Inc.

 

 

 

Tips for preparation of samples:

  • Cell concentration in each tube: about 1 million cells/ml
  • Sample volume in each tube: 0.3 - 1 ml (minimum volume is 0.3 ml)
  • Sample tubes must be polypropylene (12x75 mm round bottom polypropylene tube- BD Falcon Cat# 352002 for non sterile, or Cat# 352063 for sterile).
  • All samples should be filtered with 40 Ám nylon cell strainers (Falcon # 352340) prior to running to avoid clogging the machines.
  • If you have samples stained with more than one fluorochrome per sample, we recommend you  bring single color stained positive control samples (each fluorochrome individually) and unstained or negative control (isotype) cells for proper compensation.
  • We recommend that samples are kept on ice or chilled until ready to run on the instrument.