DOWNLOAD User Registration Form
New users need to download and fill out the User Registration Form and submit the form before/on the first appointment. This form is used for the billing information, therefore, periodically we may ask the users to update the form. A new form is needed when the grant is expired or changed.
DOWNLOAD Cell Sorting – Biosafety Assessment Form
Due to the fact that cell sorting generates aerosols and the cells are generally not fixed, the biohazard risks are greater than in analytic flow cytometry. Therefore, a Cell Sorting Biosafety Assessment Form has to be filled out prior to doing the sort. We are equipped with a BL2 biosafety cabinet to perform sorts on infectious samples, but the sorting or acquisition of potentially infectious samples must be discussed with Jessica Cervelli.
The PI/Requestor of the sort must have the appropriate biosafety approvals from the RU institutional Biosafety Committee (IBC). Material that will require IBC approval includes:
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- Unfixed primary or established human/non-human primate cell lines, and/or;
- infected/transfected/transformed cell lines (regardless of origin), and/or;
- Bacteria, viruses, parasites, fungi
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DOWNLOAD Kaluza Dongle User Agreement Form
As a condition of renting our Kaluza Dongles, all users are required to sign a User Agreement Form that we will keep on file.
Useful Links
Spectral Viewers/ Fluorochrome Tables
- FluoroFinder to build a panel for multicolor immunofluorescence
- Instrument Fluorochrome Guide – Beckman Coulter
- Invitrogen Fluorescence SpectraViewers
- Science Gateway Fluorochromes Table
Tutorials
- Introduction to Flow Cytometry- Life Sciences
- Flow Cytometry- A Basic Introduction by Michael G. Ormerod
- Kaluza Analysis Software Tutorial – Beckman Coulter
- BD Biosciences Training & E-Learning – Introduction to Flow Cytometry
- Fluorescence Tutorials – Invitrogen
- Practical Flow Cytometry (4th edition)
Software
- Kaluza Analysis Software
- Leica LAS AF Lite (version 2.3.0) software
- FlowJo
- ModFit LT, Verity Software House
General Flow Cytometry
- International Society for Advancement of Cytometry (ISAC)
- Metroflow -The NY, NJ Flow Cytometry User Group
Commercial Links