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  5. Beckman Coulter CytoFLEX Cytometer

Beckman Coulter CytoFLEX Cytometer is a compact bench-top flow cytometer for research use. The CytoFLEX flow cytometer can acquire up to 8 parameters on a single sample excited by the 488nm and 638nm lasers, up to 6 fluorescence colors, plus forward and side scatter data channels. It can be upgraded by adding a 405nm laser and up to 13 fluorescence colors in the future.

CytoFLEX Cytometer uses the sample peristaltic pump to take sample and calibrates sample volume delivery for absolute cell counts without using beads. It can process samples at flow rate between 10 uL and 240 uL per minute providing high sensitivity and adjustable flow rates.

The CytExpert software provides 7 decade of data display and the event collection rate can be up to 30,000 events/second.

Sample Input Format: 96 well-plate.

CytoFLEX Laser Specifications:

Lasers Power output
Blue laser, 488nm 50 mW
Red laser, 638nm 50 mW
Violet laser, 405nm 80 mW

CytoFLEX Configuration:

Excitation Fluorescence Channels Fluorochromes
488 nm 525/40 BP FITC, ALexa Fluor 488, CFSE, Fluo-3
585/42 BP PE, PI
690/50 BP PC5, PC5.5, PerCP, PerCP-Cy5.5, PI
780/60 BP PC7
638 nm 660/20 BP APC, Alexa Fluor 647, eFluor 660
780/60 BP APC-Alexa Fluor 750, APC-Cy7, APc-H7, APC-eFluor 780
405 nm 450/45 BP Pacific Blue, BV421, eF450, VioBlue, DAPI
525/40 BP Krome Orange, Pacific Orange, BV510,VioGreen

Location:  EOHSI Building, Room 347

Available software for analyzing data: CytExpert software allows visualization of data acquisition and data analysis of the results. CytExpert software can be installed on your desktop computer to analyze your data on your own. The data are created as FCS 3.0 format and can be analyzed using Kaluza Analysis Software or other third party software.