- Beckman Coulter CytoFLEX Cytometer
The Beckman Coulter CytoFLEX is a compact bench-top flow cytometer for research use. It can acquire 10 parameters on a single sample excited by the 405nm, 488nm, and 638nm lasers, up to 8 fluorescent colors, plus forward and side scatter data channels.
CytoFLEX Cytometer uses the sample peristaltic pump to take sample and calibrates sample volume delivery for absolute cell counts without using beads. It can process samples at flow rate between 10 uL and 240 uL per minute providing high sensitivity and adjustable flow rates.
The CytExpert software provides 7 decade of data display and the event collection rate can be up to 30,000 events/second.
Sample Input Format: 96 well-plate.
CytoFLEX Laser Specifications:
|Blue laser, 488nm||50 mW|
|Red laser, 638nm||50 mW|
|Violet laser, 405nm||80 mW|
|488 nm||525/40 BP||FITC, ALexa Fluor 488, CFSE, Fluo-3|
|585/42 BP||PE, PI|
|690/50 BP||PC5, PC5.5, PerCP, PerCP-Cy5.5, PI|
|638 nm||660/20 BP||APC, Alexa Fluor 647, eFluor 660|
|780/60 BP||APC-Alexa Fluor 750, APC-Cy7, APc-H7, APC-eFluor 780|
|405 nm||450/45 BP||Pacific Blue, BV421, eF450, VioBlue, DAPI|
|525/40 BP||Krome Orange, Pacific Orange, BV510,VioGreen|
Location: EOHSI Building, Room 347
Available software for analyzing data: CytExpert software allows visualization of data acquisition and data analysis of the results. CytExpert software can be installed on your desktop computer to analyze your data on your own. The data are created as FCS 3.0 format and can be analyzed using Kaluza Analysis Software or other third party software.