Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Fluorescence is a form of luminescence. Fluorescence microscopy allow users to determine the distribution of a single molecule species, its amount and its localization inside or outside of a cell.
The Leica DMLB upright microscope is optimized for fluorescence microscopy with a fiberoptic halide arc lamp and filter sets to detect FITC (excitation: Band pass 470/40; emission: Long pass 515), DAPI (excitation: Band pass 360/40; emission: Long pass 425) and rhodamine (excitation: Band pass 540/45; emission: Long pass 590). A separate filter for the detection of Pacific Blue (excitation: Band pass 405/30; emission: Long pass 450) is also available. The Jenoptic CF Scan digital camera and resident software allow for capture and overlap of bright field and fluorescent images.
Location: Ernest Mario School of Pharmacy, William Levine Hall, Room 002